Dr. Azhar Rafique

Associate Professor at Government College University Faisalabad

Dr. Azhar Rafique

Contact Details

Email Address azharrafique@gcuf.edu.pk
Phone Number 041-9201206
Office Address GC University Faisalabad
Academic Profiles
Basic Information
Full Name
Dr. Azhar Rafique
Designation
Associate Professor
Department
Zoology
Faculty
Life Sciences
Email Address
azharrafique@gcuf.edu.pk

Brief Statement of research work

Dr. Azhar Rafique is working as Associate Professor in the Department of Zoology Government College University, Faisalabad Pakistan, which is one of the top ranked public sector universities in Pakistan and situated in the third largest city of Pakistan previously known as Lyallpur. My job tasks include research and teaching. My research interests are in Vertebrate Pest control & Ecology, wildlife, Biodiversity, microbiology, toxicology and Parasitic diseases of public health concerns. Currently my Ph.D and M.Phil students are working on the Antimicrobial activity,Phytochemistry, Parasitic diseases, Toxicity, wildlife, and biodiversity. I also teach various undergraduate and postgraduate, and Ph.D. courses in particular Wildlife of Pakistan, Biodiversity, Research methodology, Zoogeography, Animal diversity, Principles in Animal Life, Physiology and Helminthology. I believe in team work, organization, planning and execution.

Academic Background
SR. DEGREE INSTITUTE FROM TO
1 Ph.D University of Agriculture, Faisalabad 2001 2009
2 M.Phil Or MS University of Agriculture, Faisalabad 1998 2000
3 Master Or Equivalent University of the Punjab, Lahore 1994 1997
4 Graduation Or Equivalent University of the Punjab, Lahore 1991 1994
5 Intermedite Or Equivalent BISE Faisalabad 1989 1991
6 Matriculation Or Equivalent BISE Faisalabad 1987 1989
Research Publications
SR. AUTHORS PAPER TITLE JOURNAL PAGE# YEAR VOL. IMP.F VIEW
1 Nimrah Zafar, Azhar Rafique, Shabana Naz, Muhammad Muzammil Nazir, Asma Ashraf, Unveiling the??in vitro,??in vivo??anti-inflammatory potential of??Ocimum basilicum??and??in silico??analysis of its phytocompounds targeting COXs proteins,?? Journal of Pharmacy and Pharmacology; 03 2025 3 3.2
2 Munawar Z, Amjid S, Ramzan F, Rafique A, Hassan S, Anwar U, Mehmood M, Farooq U, Khalid MF, Mustafa R, Riaz M, Rahman MA, and Abbas W Effects of partial soybean meal replacement with sunflower meal and non-starch polysaccharide degrading enzymes supplementation on broiler growth performance, nutrient digestibility, and gut morphology, Veterinary World 695-704 2025 18 0
3 Liaqat W, Anwar U, Fatima A, Rafique A, Mustafa R, Farooq U, Ramzan F, Abbas W, Khalid MF, Ashraf M, Riaz, M M., Bilal, M. Q., & Rahman, M. A. Effect of Ideal Amino Acid Ratio of Arginine to Lysine on Intake, Nutrient Digestibility, Growth Performance, Antibody Titers of Newcastle Disease and Infectious Bronchitis Disease, and Carcass Characteristics of Broilers Animals. 135 2025 15 2.7
4 Goraya MU, Shahid R, Ashraf A, Kiran S, Ain Q, Alwutayd KM, Hussain B, Hussain S and Rafique A* elenium mitigates Paraquat-Induced hematotoxicity, oxidative stress, biochemical alterations, and nephrotoxicity in male Albino rats. Pak Vet J 1361-1368 2025 45 5.4
5 Ayub I, Sani SS, Zaman A, Shahid MR, Bashir W, Ijaz M, Zafar S and Rafique A*, Prevalence of Toxoplasma gondii in humans and animals and its economic impacts. Pak Vet J 1-9 2025 45 5.4
6 Nawaz Y, Mahmood MS, Wazir I, Khan A, Ullah Q, Rafique A, Shahid M, Alzahrani KJ, Alsharif KF, Nawaz S. Seroprevalence of Bovine Brucellosis in Cattle in District Bannu, Khyber Pakhtunkhwa, Pakistan. Polish Journal of Microbiology 416-427 2025 74 1.6
7 Khan MK, Mahmood MS, Ali S, Khatoon A, Umar S, Mughal MAS, Azeem A, Chatha AK, Abbas Z and Rafique A*, Prevalence and characterization of Buxtonella sulcata in bovines in Pakistan using morphological and PCR-based approaches. Pak Vet J 1298-1302 2024 44 3.8
8 Masoud F, Ashraf MA, Usmani MW, Rafique A, and Aslam R, Newcastle disease virus as a viral vector platform for poultry vaccines Pak Vet J, 539-546 2024 44 3.8
9 Naz, S., Raza, N., Alhidary, I., Satti, S., Rafique, A., Batool, S., ??? Khan, R. U. Evaluation of copper nanoparticles on growth, organs histology and DNA damage in Japanese quails (Coturnix coturnix japonica). Toxin Reviews 127-136 2024 43 0
10 Shakir MZ, Rizvi F, Masoud F, Rafique A*, Usmani MW, Naseem N and Usman M, Seroprevalence, molecular confirmation and application of bacteriophage as a potential prophylactic therapy against fowl typhoid disease in commercial poultry birds. Pak Vet J 1185-1192 2024 44 3.8
11 Shabana Naz, Azhar Rafique, Asma Ashraf, Sajida Batool, Ibrahim A. Alhidary & Shamsuddin Shamsi Haematological alterations under the anti-helminthic application in Pavo cristatus, Journal of Applied Animal Research 630-636 2023 51 1.987
12 Ghazwani M, Hakami AR, Sani SS, Sultana S, Sultana T, Bashir W and Rafique A*, Antibacterial activity of aqueous and methanolic extract of Mentha piperita against pervasive bacteria isolated from Urial the Ovis vignei Pak Vet J 103-108 2023 43 2.3
13 Muhammad Umar Ijaz, Ayesha Ishtiaq, Arfa Tahir, Mughees Aizaz Alvi, Azhar Rafique, Peng Wang and Guo-ping Zhu Antioxidant, anti-inflammatory, and anti-apoptotic effects of genkwanin against aflatoxin B1-induced testicular toxicity, Toxicology and Applied Pharmacology, 116750 2023 481 4.46
14 Masoud, F, M. S. Mahmood, R. Z. Abbas, H. M. N. Cheema, A. Rafique,* S. Ali, R. Aslam, M. K. Khan Engineered Newcastle disease virus expressing the haemagglutinin protein of H9N2 confers protection against challenge infections in chickens. Process Biochemistry 281-290 2023 130 4.885
15 ALRashdi BM, Germoush MO, Sani SS, Ayub I, Bashir W, Hussain B, Mazhar M, Ali S, Zahid Z, Ayesha S and Rafique A* Biosynthesis of Salvia hispanica based Silver nanoparticles and evaluation of their antibacterial activity in-vitro and rat model Pak Vet J 283-289 2023 43 2.3
16 Ahmad Zaman, Azhar Rafique*, Asma Ashraf, Muhammad Shahid Mahmood, Nazia Nahid, Farhat Jabeen, Salma Sultana, Tayyaba Sultana, Sultan Ali, S. M. Neamul Kabir Zihad, and Shaikh Jamal Uddin. Diversity, Abundance, and Distribution of Avifauna in District Jhang, Pakistan. Journal of Zoological Systematics and Evolutionary Research 3308651 2023 2023 2.424
17 Asad, F, A. Ashraf, A. Rafique, S. Qamera, S. Naza, T. Alia, Z. Shaheena and A. Mazhar. 19.Chromium supplemented carbohydrate diets for Cirrhinus mrigala: effects on body composition, gut enzyme activity and hematological parameters. Brazilian Journal of Biology, 6984 2023 83 .401
18 Mahmood, M.S, A. Ashraf, S. Ali, A.B. Siddique, F. Asad, R.Z. Abbas, F. Siddique, A. Aslam, R. Aslam and A. Rafique* Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation. Brazilian Journal of Biology, 6984 2023 83 .401
19 Zaman, A., A. Rafique*, F. Jabeen and T. Sultana, Diversity, Abundance and Seasonal Assessment of Wild Birds in Urban Habitat of District Chiniot, Pakistan. Pakistan Journal of Zoology, 525-534 2023 55 .6
20 Rafique, A*. Mahmood, M. S., Abbas, R. Z., Ali, S., Mian, A., & Rizwan, A. Anthelmintic activity of Moringa oleifera and Azadirachta indica against gastrointestinal nematodes of wild sheep Journal of the Hellenic Veterinary Medical Society, 3989-3996 2022 73 .516
21 Masih, A., A. Rafique*, A.B. Siddique, et al. Babesia bovis in Large Ruminants in Pakistan - Molecular Detection and Haemato-Biochemical Alterations Acta Scientiae Veterinariae. 1890 2022 50 .3
22 Rafique A*,, Nasir S, Ashraf A, Nawaz Z, Zahid FM, Abbas A and Masood S, Serosurveillance and risk factors analysis of caprine toxoplasmosis in Faisalabad Punjab, Pakistan. Pak Vet J 102-106 2022 42 1.308
23 Masih, A., A. Rafique*, F. Jabeen and S.Naz Detection of Babesia ovis in small ruminants by using microscopic and molecular techniques Pak. J. Agri. Sci., 511-517 2022 59 .748
24 Zaman MA, Rafique A, Mehreen U, Mehnaz S, Atif FA, Abbas A, Hussain K, Raza MA, Altaf S, Siddique F, Masudur RMd, Omar M Epidemiological investigation and development of loop mediated isothermal amplification for the diagnosis of ovine theileriosis Pak Vet J 370-375 2022 42 1.803
25 Ishaq K, Ahmad A, Rafique A, Aslam R, Ali S, Shahid MA, Sarwar N, Aslam MA, Aslam B, Arshad MI Occurrence and antimicrobial susceptibility of Proteus mirabilis from chicken carcass. Pak Vet J, 576-579 2022 42 .831
26 Abbas R, Nawaz Z, Siddique AB, Aslam R, Rafique A, Zahoor MA, Qamar MU, Ahmad MZ, Jalees MM, Qasim M and Alsayeqh AF, Molecular detection of biofilm production among multidrug resistant isolates of Pseudomonas aeruginosa from meat samples. Pak Vet J, 505-510 2022 42 1.803
27 Rafique A.,*, M. S. Mahmood , R.Z. Abbas , A. Ashraf , S. Nasir , F. Jabeen , T. Sultana , S. Sultana , G. Abbas, M. Luqman , A.Abbas and S. F. Abbas Albaayit ANALYSIS OF DIFFERENT FACTORS CONTRIBUTING THE RODENT INFESTATIONS IN URBAN AREAS IN FAISALABAD, PAKISTAN Pak. J. Agri. Sci 1197-1203 2021 58 .748
28 Arshad, M., S. M. Hussain, A. Rafique, F. Ahmad, M. D. Shahzad, I. Ucak, A. Javid, H. Azmat, A. I. Hussain, A. Sharif and M. Asrar. Effect of ??-tocopherol supplementation on growth performance, antioxidant activity and nutrient digestibility of Labeo rohita fingerlings fed corn gluten meal- based diet. Indian Journal of Fisheries, 86-94 2021 68 .5
29 Khan. M, M. Sharif, M. F. Naeem, H. Munir, A. Rafique , M. F.A. Chishti , U. Anwar, Q. Bilal , M. Riaz , M. Hussain, S. Imran, F.Hussain and M.Aziz Ur Rahman. EFFECT OF Lactobacillus plantarum AND Lactobacillus buchneri ON COMPOSITION, AEROBIC STABILITY, TOTAL LACTIC ACID BACTERIA AND E. COLI COUNT OF ENSILED CORN STOVER WITH OR WITHOUT MOLASSES SUPPLEMENTATION Pak. J. Agri. Sci., 677-784 2021 58 .748
30 Masih, A., A. Rafique*, F. Jabeen and S.Naz Molecular Epidemiology of Bovine Babesiosis in Punjab, Pakistan Acta Scientiae Veterinariae 1-10 2021 49 .37
31 Yousaf, I. , S. Nasir,, S. M. Hussain, A. Rafique , M.U. Ijaz , M. Asrar , S.Rehman, B. Ahmad, N. A. Khan and M. Iftikhar THE MOSQUITO MICROBIOME: ANALYSIS FOR ENDEMIC AND EMERGING PATHOGENS OF PUBLIC HEALTH SIGNIFICANCE IN DISTRICT FAISALABAD, PAKISTAN. Pak. J. Agri. Sci., 161-167 2021 58 .748
32 Nawaz Z, Aslam B, Zahoor MA, Siddique AB, Rafique A, Aslam R, Qamar MU, Ali S, Mubeen MU, Frequency of Extended Spectrum Beta Lactamase Producing Escherichia coli in Fresh and Frozen Pak Vet J 102-106 2021 41 1.318
33 Andleeb R, M. U. Ijaz, A. Rafique, A. Ashraf, N. Bano, N. Zafar, F. Tasleem, R. A. Marc (Vlaic) , O. L. Pop and H. T. Ahmedah. Biological Activities of Methanolic Extract of Aegle marmelos against HN Protein of Newcastle Disease Virus.Agronomy 1784 2021 11 3.417
34 Lin X, Rafique A, Fayyaz T, Bashir W, Luqman M, Zahid FM and Zhou K Appraisal of Cymbopogon Citratus (Lemon grass) for antibacterial activity against Uropathogens Pak Vet J 122-126 2021 41 1.318
35 Liu, J, M. S. Mahmood , R. Z.Abbas , A. Dillawar, Z. Nawaz , M. Luqman , A. Abbas, A.Rehman and A. Rafique THERAPEUTIC APPRAISAL OF ETHANOLIC AND AQUEOUS EXTRACTS OF CLOVE (Syzygium aromaticum) AND GARLIC (Allium sativum) AS ANTIMICROBIAL AGENT. Pak. J. Agri. Sci. 245-251 2021 58 .748
36 Siddique,F., R. Z. Abbas, A. Iqbal, M .Rbbani, A. Rafique, I. Hussain, R. Ahmed, M. S Mahmood, A.Lotf. Development of Humoral Immune Response to Thermostable Newcastle Disease Vaccine Strain I-2 in Ring-Necked Pheasant (Phasianus colchicus) Kafkas Universitesi Veteriner Fakultesi Dergis 253-258 2021 27 .685
37 Tanweer,A., S. Naz, A, Rafique A, Ashraf, A Prevalence of Endo-parasites in Peafowls (Pavo cristatus) under Captivity Stress and Ameliorative Effect of Anti-Helminthic Drugs. Pakistan Journal of Zoology 627-633 2021 53 .85
38 Hafeez, M.A., Sattar, A., Ashraf, K., Mehdi, M., Rafique, A., Mahmood, M.S., Aslam, F., Hussain, I. and Zia, M.R., Effect of Ibuprofen Alone and in Conjugation with Vitamine and selenium on experimentally Induced Coccidiosis in Broliers. The J. Anim. Plant Sci 627-633 2020 30 .529
39 Farooq R, Hafeez MA, Oneeb M, Rafique A Ashraf K, Rauf N, Khalid K, Bilal F and Sattar A, Molecular characterization and phylogenetic analysis of Babesia species isolated from domestic cattle. Pak.vet. J 224-228 2020 40 1.175
40 Abbas RZ, Zaman MA, Sindhu ZUD, Sharif M, Rafique A, Saeed Z, Rehman TU, Siddique F, Zaheer T, Khan MK, Akram MS, Chattha AJ, Fatima U, Munir T and Ahmad M, Anthelmintic effects and toxicity analysis of herbal dewormer against the infection of Haemonchus contortus and Fasciola hepatica in goat Pak Vet J, 455-460 2020 40 1.175
41 Rafique*A., Luqman M, Zeeshan N, Ashraf,A, Nasir S, Albaayit SFA, Mahmood MS, Abbas RZ, Jabeen F, Sultana T, Sultana S, Naz, S and Asad F, MULTIPLEX PCR BASED DETECTION OF TOXIN PRODUCING Bacillus cereus FROM DIFFERENT MILK SAMPLES RETAILED IN PAKISTAN. Pak. J. Agri. Sci. 887-891 2020 57 .667
42 Rafique A, Mahmood MS, Ashraf A, Nasir S, Kamran M, Jamil N, Jabeen F, Sultana T, Sultana S, Khan JA, Luqman M and Shamim A, Phenotypic and genotypic assortment of Polyhydroxyalkanoates producing bacteria from rumen flora of domesticated animals in Pakistan. Pak Vet J 88-92 2020 40 1.175
43 Asad F, Qamer S, Ashraf A, Rafique A, Shaheen Z, and Nisar A, Apparent nutrient digestibility of carbohydrate (corn) in Cirrhinus mrigala under the influence of chromium chloride hexahydrate. Brazilian Journal of Biology 518-522 2020 80 1.26
44 MAHMOOD, M. S., A. RAFIQUE,*W. YOUNAS, AND B. ASLAM Momordica Charantia L.(bitter gourd) as a candidate for the control of bacterial and fungal growth Pak. J. Agri. Sci. 1031-1036 2019 56 .677
45 HUSSAIN R, F. ALI , A. RAFIQUE , A. GHAFFAR, G. JABEEN, M. RAFAY ,S. LIAQAT ,I. KHAN, R. MALIK, M.K. KHAN,M. NIAZ , K. AKRAM AND A. MASOOD . Exposure to sub-acute concentrations of glyphosate induce clinicohematological, serum biochemical and genotoxic damage in adult cockerels Pak Vet J, 181-186 2019 39 1.36
46 Batool M, Nasir S, A. Rafique, I. Yousaf and M. Yousaf. Prevalence of tick infestation in farm animals from Punjab, Pak Vet J. 406-410 2019 39 1.36
47 SHAHZAD, S., M.A. ASHRAF, M. SAJID, A. SHAHZAD, A. RAFIQUE. AND M.S. MAHMOOD. 2018 . Evaluation of synergistic antimicrobial effect of vitamins (A, B1, B2, B6, B12, C, D, E and K) with antibiotics against resistant bacterial strains. Journal of Global Antimicrobial Resistance 231-236 2018 13 2.022
48 SIDDIQUE, A. B., Z. NAWAZ, M. A. ZAHOOR, A. RAFIQUE, B. ASLAM, N. NAZIA AND R. HUSSAIN. 2018 Efficacy of Lytic Bacteriophages against Multidrug Resistant Salmonella enteritidis from Milk and Meat Pak. Vet. J 353-358 2018 38 1.392
49 ISHAQ, M.U., A. RAFIQUE, H.M.N. CHEEMA, M. U. ASHRAF, S.U. RAHMAN, R. Z. ABBAS AND M. S. MAHMOOD. 2018. Role of cytosine-phosphate-guanosine Oligodeoxynucleotides (CpG ODNs) as adjuvant in poultry vaccines. World's Poultry Science Journal, 1-10 2018 74 1.439
50 MAHMOOD, M.S., H. W. AMIR, R.Z. ABBAS, B. ASLAM AND A. RAFIQUE Evaluation of Antiviral Activity of Azadirachta indica (Neem) Bark Extract against Newcastle Disease Virus. Pakistan Vet. J 25-28 2018 38 1.392
51 4. RAFIQUE, A, F. IQBAL, A. ASHRAF, F. JABEEN, S. NAZ AND M. S. MAHMOOD Seroprevalence of Toxoplasma gondii and its effect of hematological picture in commensal rodents in Faisalabad Pakistan. Pak. J. Agri. Sci 195-199 2017 54 1.054
52 3. ASHRAF, M. U., M. S. MAHMOOD, A. RAFIQUE, R. Z. ABBAS, Z. IQBAL, M. YOUNAS, S. A.SADIQ, M. USMAN1, M. O. ASGHAR AND M. U. ISHAQ. Factors responsible for the continuous persistence and evolution of low pathogenic avian influenza virus (H9N2) World's Poultry Science Journal 791-802 2017 73 1.037
53 2. AHMAD, T, S. NASIR, I. NASIR, T. NAWAZ, A. RAFIQ AND I. YOUSAF. Response Surface Modeling for West Nile Viral Encephalitis Mosquito Control Experiments. Pakistan . Pakistan Vet. J. 465-469 2017 37 .813
54 ASHRAF, A., M. M. ASHRAF, A. RAFIQE, B. ASLAM, S. GALANI, S. ZAFAR, F. ASAD, R. D. ASGHAR, S. AKRAM, H. AHMED, S. M. ALI SHAH AND R. ASIF In vivo antiviral potential of Glycyrrhiza glabra extract against Newcastle disease disease Pak. J. Pharm. Sci 565-572 2017 30 .586
55 AZHAR RAFIQUE ANTIVIRAL EFFECTS OF GREEN TEA (CAMELLIA SINENSIS) AGAINST PATHOGENIC VIRUSES IN Afr J Tradit Complement Altern Med 176-184 2016 13 .88
56 MAHMOOD, M.S., M.F. AHMAD, I. HUSSAIN, R. Z. ABBAS, A. KHAN AND A. RAFIQUE Growth promoting effect of Pimpinella anisum (Aniseed ) in Broiller chickens Bullt. Latino.Am.Car. P. Med. Aro.(Blacpma). 278-284 2014 13 .636
57 7. MAHMOOD, M.S., S. ALI, I. HUSSAIN, A. ASLAM AND A. RAFIQUE, Development of hydropericardium syndrome vaccines (from beginning to present vaccine) (a review World???s Poultry Science Journal 355-364 2014 70 1.36
58 SIDDIQUE, F., I. HUSSAIN, M.S. MAHMOOD, A. RAFIQUE AND A. IQBAL Descriptions of alpha amylase produced from Aspergillus niger. Pakistan Journal of Science 140-144 2014 66 .27
59 MAHMOOD, M.S., F. SIDDIQUE, I. HUSSAIN, S.I. AHMAD, A. RAFIQUE AND A. IQBAL Thermostable Vaccines for Newcastle Disease: A Review. World???s Poultry Science Journal 829-838 2014 70 1.36
60 MAHMOOD, M.S, I. HUSSAIN, M. F. AHMAD, A. KHAN, R. Z. ABBAS, A. RAFIQUE, Immunomodulatory effects of Pimpinella anisum L. (Aniseed) in Broiler Chicks against Newcastle Disease and Infectious Bursal Disease Viruses . Bullt. Latino. Am. Car. P. Med. Aro. (Blacpma). 415-422 2014 13 .636
61 11. KHAN, A., M. S. MAHMOOD, I. HUSSAIN, F. SIDDIQUE, A. RAFIQUE, A. IQBAL AND R. Z. ABBAS Bacteriological and Epidemiological Investigations of Pullorum Disease in Selected Poultry Farms of Faisalabad, Pakistan. Global Veterinaria 455-460 2014 12 .6
62 HUSSAIN, I., M.S. MAHMOOD,M. I ARSHAD, M. AKHTAR AND A. RAFIQUE Immune system dysfunction in broiler chickens experimentally inoculated with Fowl adenovirus serotype-4 associated with inclusion body hepaptitis hydropericardium syndrome (IBH-HPS). Turkish J. Vet. Animal Sci 223-230 2012 36 .236
63 RAFIQUE, A., S. A. RANA, H.A. KHAN AND A. SOHAIL, Prevalence of some heliminths in rodents captured from different city structures including poultry farms and human population of Faisalabad, Pakistan . Pakistan Vet J 141-144 2009 29 0
64 15. MUSTHTAQ-UL-HASSAN, M., I. HUSSAIN, B, SHAHZADI, M. SHAHEEN, M. S. MAHMOOD, A. RAFIQUE AND M. MAHMOOD-UL-HASSAN, Occurrence of some zoonotic microorganisms in feacal matter of house rat (Rattus rattus) and house mouse (Mus musculus) trapped from various structures. Pakistan Vet. J 171-174 2008 28 0
65 RAFIQUE A AND S.A. RANA, Species association of some earthworms in the Agro-Ecosystem of Faisalabad and Sargodha Pak. J. Agri. Sci 27-28 2001 38 0
66 RANA, S. A., A. RAFIQUE AND J. I. QURESHI Ecological distribution of earthworms species along some water channels, water ditches, canals and river in the Agro-Ecosystem of Faisalabad Division JAPS 126-130 2000 10 0
Professional Journey

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International Exposure

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Professional qualifications & traninings
SR. INSTITUTE CERTIFICATE YEAR MAJOR SUBJECT
1 Education college, Faisalabad B.ED 1997 - 1998 Biology, chemistry,
Research Projects

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