Dr. Muhammad Shafique

Approved Supervisor

Associate Professor at Government College University Faisalabad

Dr. Muhammad Shafique

Contact Details

Email Address drmshafique@gcuf.edu.pk
Phone Number 041-9203023
Office Address GC University Faisalabad
Academic Profiles
Basic Information
Full Name
Dr. Muhammad Shafique
Designation
Associate Professor
Department
Institute of Microbiology
Faculty
Life Sciences
Email Address
drmshafique@gcuf.edu.pk

Brief Statement of research work

Academic Excellence

Academic Background
SR. DEGREE INSTITUTE FROM TO
1 Ph.D Other University 2008 2013
2 M.Phil Or MS Quaid-i-Azam University, Islamabad 2005 2007
3 Master Or Equivalent University of Agriculture, Faisalabad 1993 1998
4 Intermedite Or Equivalent BISE Lahore 1990 1992
5 Matriculation Or Equivalent BISE Lahore 1988 1990
Research Publications
SR. AUTHORS PAPER TITLE JOURNAL PAGE# YEAR VOL. IMP.F VIEW
1 Parveen, N., Shafique, M., Aslam B. and Khurshid M. Diabetes Mellitus and Enhanced Vulnerability to Escherichia coli Catheter Associated Urinary Tract Infections: Integrative Clinical and Molecular Analysis Polish Journal of MIcrobiology 084-095 2026 75 1.6
2 Shafique, M., Ghori, M. U., Hassan, M., Ashfaq U. A. and Masoud, M.S. Network pharmacology and machine learning reveal multi-target mechanisms of poly herbal formulation against atherosclerosis Pakistan Journal of Pharmaceutical Sciences 1578-1589 2026 39 .6
3 Mustafa, A.U., Rasool, M.H., Aslam, B., and Shafique, M. Performance of Rapid Antigen Tests and Inflammatory Markers in SARS-CoV-2: Lessons for Future Respiratory Pandemics Journal of Liaquat University of Medical & Health Sciences 1-16 2026 25 .2
4 Nauman, M., Shafique, M., Aslam, B., Khurshid, M. Public health challenges of tuberculosis in urban slums: Prevalence and drug resistance in Punjab, Pakistan Acta Microbiologica et Immunologica Hungarica 101-109 2026 73 1.6
5 Ahmed, H., Nazir, F., Malik, I.R., Naseem, N., Shafique, M., Ansari, R., Khan, A.A.U., Sajid, F., Shaheen, G., Quddoos, M.Y., Ahmad, T., Abid, A., Rahmat, S.A., Naqvi, S.M. and Akram, M. Hepatoprotective Activity of Medicinal Plants Multidisciplinary Surgical Research Annals 1348-1354 2025 3 0
6 Sarwar, A.; Aslam, B.; Rasool, M.H.; Shafique, M.; Khurshid, M.; Sasanya, J.; Aljasir, S.F. Genetic Diversity and Antibiotic Resistance Paradigm of Enterobacterales in Animal-Derived Food Sources: A One Health Disquiet Pathogens 1040 2025 14 3.3
7 Abbas, F.A., Batool, S., Malik, I.R., Abbas, A., Nawaz, Z., Shafique, M. & Muniiru, L. Lumpy skin disease in Pakistan: unraveling the outbreak and charting future horizons NUST Journal of Natural Sciences 1-16 2025 10 0
8 Qamar M.U., Fizza K., Chughtai M.I., Shafique M., Seytkhanova B., Yktiyarov A., Aatika, Saleem Z., Mustafa S., Tufail Z., Chaudhry M., Shah T.A., Salamatullah A.M., Bourhia M Food Safety Concerns in Pakistan: Monitoring of Antimicrobial-Resistant Bacteria and Residue Contamination in Commercially Available Fish and Poultry Meat Sample Foodborne Pathogens and Disease 841-855 2025 22 1.9
9 Ghori, M. U., Masoud, M. S., Shafique, M., Hussain, M., & Awan, F. R. Association of obesity traits and uric acid levels with cardiovascular disease in Punjabi patients from Faisalabad, Pakistan Acta Cardiologica 465-474 2025 80 2.1
10 Ghori MU, Masoud MS, Shafique M, Fiaz H, Hussain M, Awan FR. A Comparative Study of Obesity-Related Traits and Serum Lipid Parameters in Cardiovascular Patients from Faisalabad, Pakistan Makara Journal of Health Research 17-24 2025 29 0
11 Qureshi M.H.F., Azam F., Shafique M., Aslam B., Farooq M., Rehman A.U., Rafique M.K., Meraj M.T. and Ahmed I. A One Health Perspective of Pet Birds Bacterial Zoonosis and Prevention PAKISTAN VETERINARY JOURNAL 1-8 2024 44 2.3
12 Mudassar, M., Shafique, M.*, Rasool, M.H., Aslam. B. and Shamsi, F.B. Half-life determination of COVID-19 antibodies among vaccinated and non-vaccinated people Journal of Population Therapeutics and Clinical Pharmacology 710-720 2024 31 1.94
13 Nawaz Z, Zahoor MK, Shafique M, Athar R, Yasmin A and Zahoor MA In vitro assessment of probiotic properties of lactic acid bacteria isolated from camel milk: enhancing sustainable foods Frontiers in Sustainable Food Systems 1-7 2024 8 4.69
14 Hussain, M., Ghori, M.U., Aslam, M.N., Abbas, S., Shafique, M. and Awan, F.R. Serum uric acid: an independent risk factor for cardiovascular disease in Punjabi Pakistani patients BMC Cardiovascular Disorders 546 2024 24 2.13
15 Aslam B., Asghar R., Muzammil S., Shafique M., Siddique A.B., Khurshid M., Ijaz M., Rasool M.H., Chaudhry T.H., Aamir A. and Baloch Z AMR and Sustainable Development Goals: at a crossroads Globalization and Health 1-21 2024 20 5.9
16 Bashir, I., Rasool, M.H., Shafique, M., Jabeen, K. and Qamar, M.U. Exploring the antimicrobial efficacy of Manuka honey against multidrug-resistant and extensively drug-resistant Salmonella Typhi causing septicemia in Pakistan Future Microbiology 1377-1387 2024 19 2.76
17 Shafique M, Shoaib I, Aslam B, Khalid R, Tanvir I, Rasool MH, Shah TA, Almaary KS, Bourhia M, Qamar MU. Detection of high-risk human papillomavirus infected cervical biopsies samples by immunohistochemical expression of the p16 tumor marker Archives of Microbiology 1-8 2024 206 2.95
18 Shafique M., Khurshid M., Muzammil S., Arshad M.I., Malik I.R., Rasool M.H., Khalid A., Khalid R., Asghar R., Baloch Z. and Aslam B. Traversed dynamics of climate change and One Health Environmental Sciences Europe 135 2024 36 5.84
19 Malik, I.R., Shafique, M., Raza, M., Ahmad, W., Shahbaz, Y., Yousafzai, A., Umar, M. and Asif, M. Monkeypox virus recent breakthrough: review on efficacy of diagnosis and treatment methods Pak-Euro Journal of Medical and Life Sciences 267-282 2024 7 .119
20 Tariq MU, Muzammil S, Ashfaq UA, Arshad MI, Shafique M, Ejaz H, Khurshid M, Eltayeb LB, Mazhari BBZ, Elamir MYM, Al-Harthi HF, Rasool MH, and Aslam B. Characterizing the bacteriophage PKp-V1 as a potential treatment for ESBL-producing hypervirulent K1 Klebsiella pneumoniae ST258 isolated from veterinary specimens Veterinary World 2008-2016 2024 17 1.7
21 MM Sajid, NA Shad, Y Javed, M Shafique, AM Afzal, SB Khan, N Amin, ... Efficient photocatalytic and antimicrobial behaviour of zinc oxide nanoplates prepared by hydrothermal method Journal of Cluster Science, 1-11, 2022 1-11 2022 0
22 SAJID M.M., SHAD N.A., AFZAL A.M., SHAFIQUE, M., AFZAL A.M., KHAN, S.B., AMIN, N., HASSAN, M.A., KHAN M.U.H., TARABI, T. AND ZHAI, H. Efficient Photocatalytic and Antimicrobial behaviour of Zinc Oxide Nanoplates Prepared by Hydrothermal Method Journal of Cluster Science 773-783 2022 33 3.061
23 B Aslam, MH Siddique, AB Siddique, M Shafique, S Muzammil, ... Distribution of mcr-1 Harboring Hypervirulent Klebsiella pneumoniae in Clinical Specimens and Lytic Activity of Bacteriophage KpnM Against Isolates Infection and Drug Resistance, 5795-5811, 2022 5795-5811 2022 0
24 SHAH, A.A., AHMAD, I., SHAFIQUE, M., SIDDIQUE, A.B., ASLAM, B. AND QAMAR M.U. Antibacterial activity of Silver nanoparticles against carbapenem resistant Acinetobacter baumannii clinical isolates Pakistan Journal of Pharmaceutical Sciences 203-208 2022 35 .684
25 MOEED AHMAD, ABU BAKER SIDDIQUE, SAIMA MUZAMMIL, MUHAMMAD SHAFIQUE, ZEESHAN NAWAZ, MOHSIN KHURSHID, MUHAMMAD HIDAYAT RASOOL, MUHAMMAD MOAZAM JALEES, NOREEN SARWAR AND BILAL ASLAM Occurrence of Hypervirulent Klebsiella pneumoniae in Clinical Settings and Lytic Potential of Bacteriophages Against the Isolates JUNDISHAPUR JOURNAL OF MICROBIOLOGY 1-11 2022 15 .747
26 ZAFAR M.A., SHAFIQUE, M.*, ZAHOOR, M.A., SAQALEIN, M., ASLAM, B. ARSHAD, M.I., WAHEED, U. AND KASHIF, M. Evaluation of seroprevalence and associated risk factors of Toxoplasmosis in sheep and goats in District Jhang-Pakistan Journal of the Hellenic Veterinary Medical Society 3881-3888 2022 73 .477
27 MM Sajid, H Zhai, NA Shad, M Shafique, AM Afzal, Y Javed, SB Khan, ... Construction of 1T-MoS 2 quantum dots-interspersed (Bi 1??? x Fe x) VO 4 heterostructures for electron transport and photocatalytic properties RSC advances 11 (22), 13105-13118, 2021 13105-13118 2021 11 0
28 MM Sajid, H Zhai, NA Shad, M Shafique, AM Afzal, Y Javed, SB Khan, ... Photocatalytic performance of ferric vanadate (FeVO4) nanoparticles synthesized by hydrothermal method Materials Science in Semiconductor Processing 129, 105785, 2021 105785 2021 129 0
29 R Qureshi, MU Qamar, M Shafique, S Muzammil, MH Rasool, I Ahmad, ... Antibacterial efficacy of silver nanoparticles against Metallo-??-lactamase (blaNDM, blaVIM, blaOXA) producing clinically isolated Pseudomonas aeruginosa Pakistan Journal of Pharmaceutical Sciences 34 (1), 237-243, 2021 237-243 2021 34 0
30 H Qurat-ul-Ain, M Ijaz, AB Siddique, S Muzammil, M Shafique, MH Rasool, ... Efficacy of phage-antibiotic combinations against multidrug-resistant Klebsiella pneumoniae clinical isolates Jundishapur Journal of Microbiology 14 (1), 1-7, 2021 1-7 2021 14 0
31 MU Qamar, A Ambreen, A Batool, MH Rasool, M Shafique, A Khan, ... Molecular Detection of Extensively Drug-Resistant Salmonella Typhi and Carbapenem-Resistant Pathogens in Pediatric Septicemia Patients in Pakistan ??? a Public????? Future Microbiology 16 (10), 731-739, 2021 731-739 2021 16 0
32 RABIA QURESHI, MUHAMMAD USMAN QAMAR, MUHAMMAD SHAFIQUE, SAIMA MUZAMMIL, MUHAMMAD HIDAYAT RASOOL, IJAZ AHMAD, HASAN EJAZ Antibacterial efficacy of silver nanoparticles against metallo-?-lactamase (blaNDM, blaVIM, blaOXA) producing clinically isolated Pseudomonas aeruginosa Pakistan Journal of Pharmaceutical Sciences 237-243 2021 34 .684
33 NAWAZ, Z., SHAFIQUE, M., ZAHOOR, M.A., SIDDIQUE, A.B., ALI, S., ARSHAD, R., KAUSAR, S., KHAN, K., ASAD, M., REHMAN, A.U., MASIH, I. Sero-epidemiology and risk factor analysis of human brucellosis in Punjab, Pakistan: a cross sectional study Tropical Biomedicine 413-419 2021 38 .623
34 QAMAR M.U., AMBREEN, A., BATOOL, A., RASOOL, M.H., SHAFIQUE, M., KHAN, A., NISAR, M.A., KHALID, A., JUNAID, K., ABOSALIF, K.O.A. AND HASAN EJAZ, H. Molecular detection of extensively drug-resistant Salmonella Typhi and carbapenem-resistant pathogens in pediatric septicemia patients in Pakistan ? a public health concern Future Microbiology 731-739 2021 16 3.165
35 QURAT-UL-AIN, H., IJAZ, M., SIDDIQUE, A.B., MUZAMMIL, S., SHAFIQUE, M., RASOOL, M.H., KHURSHID, M., CHAUDHRY, T.H. AND ASLAM, B Efficacy of phage-antibiotic combination against MDR Klebsiella pneumoniae clinical isolates JUNDISHAPUR JOURNAL OF MICROBIOLOGY e111926 2021 14 .747
36 SAJID M.M., ZHAI H., SHAD N.A., SHAFIQUE, M., AFZAL A.M., JAVED Y., KHAN S.B., IKRAM M., AMIN N., ZHANG Z. Photocatalytic Performance of Ferric Vanadate (FeVO4) Nanoparticles Synthesized by Hydrothermal Method Materials Science in Semiconductor Processing 105785 2021 129 3.927
37 SAJID M.M., ZHAI H., SHAD N.A., SHAFIQUE, M., AFZAL A.M., JAVED Y., KHAN S.B., AMIN N., ZHANG Z. Construction of 1T-MoS2 quantum dotsinterspersed (Bi1-xFex) VO4 heterostructures for electron transport and photocatalytic properties RSC Advances 13105-13118 2021 11 3.361
38 M Usman Qamar, B S Lopes, B Hassan, M Khurshid, M Shafique, ... The present danger of New Delhi metallo-??-lactamase: A threat to public health Future microbiology 15 (18), 1759-1778, 2020 1759-1778 2020 15 0
39 B Aslam, M Rasool, S Muzammil, AB Siddique, Z Nawaz, M Shafique, ... Carbapenem resistance: Mechanisms and drivers of global menace Pathog Bact, 1-11, 2020 1-11 2020 0
40 S Sabir, MA Zahoor, M Waseem, MH Siddique, M Shafique, M Imran, ... Biosynthesis of ZnO Nanoparticles Using Bacillus Subtilis: Characterization and Nutritive Significance for Promoting Plant Growth in Zea mays L Dose-Response 18 (3), 1559325820958911, 2020 1559325820958911 2020 18 0
41 SUMERA SABIR , MUHAMMAD ASIF ZAHOOR, MUHAMMAD WASEEM, MUHAMMAD HUSSNAIN SIDDIQUE, MUHAMMAD SHAFIQUE, MUHAMMAD IMRAN, SUMREEN HAYAT, IMRAN RIAZ MALIK, SAIMA MUZAMMAL Biosynthesis of ZnO Nanoparticles Using Bacillus Subtilis: Characterization and Nutritive Significance for Promoting Plant Growth in Zea mays L Dose-Response 1559325820958911 2020 18 2.438
42 MUHAMMAD SIDDIQUE, MUHAMMAD SHAFIQUE, ABDUL MATEEN, NAVEED SHAHZAD, BILAL ASLAM, NASIR AHMAD AND MUHAMMAD USMAN QAMAR Prevalence and genotypic analysis of Hepatitis C virus isolated from Liver Center, Faisalabad, Pakistan Pakistan Journal of Zoology 313-319 2020 52 .79
43 MUHAMMAD USMAN QAMAR, BRUNO S LOPES, BREKHNA HASSAN, MOHSIN KHURSHID, MUHAMMAD SHAFIQUE, MUHAMMAD ATIF NISAR, MASHKOOR MOHSIN, ZEESHAN NAWAZ, SAIMA MUZAMMIL, BILAL ASLAM, HASAN EJAZ, MARK A TOLEMAN The present danger of New Delhi metallo-?-lactamase: a threat to public health Future Microbiology 1759-1778 2020 15 2.907
44 MUHAMMAD HIDAYAT RASOOL, MUHAMMAD ZAHEER, MUHAMMAD FAROOQUE HASSAN, MUHAMMAD SHAFIQUE AND MUHAMMAD USMAN QAMAR Isolation and antimicrobial susceptibility paradigm of carbapenem resistant metallo-beta-lactamase producing Gram negative rods Pakistan Journal of Zoology 849-854 2019 51 .79
45 ASIF MEHMOOD, MUHAMMAD HIDAYAT RASOOL, AFTAB AHMAD ANJUM, MUHAMMAD ASIF ZAHOOR AND MUHAMMAD SHAFIQUE Pathotyping and Genetic Characterization of a Highly Virulent Newcastle Disease Virus Strain Isolated from Recent Outbreak in District Okara, Pakistan PAKISTAN VETERINARY JOURNAL 353-358 2019 39 1.36
46 M Shafique, MA Zahoor, MI Arshad, B Aslam, AB Siddique, MH Rasool, ... Hurdles in Vaccine Development Against Respiratory Syncytial Virus The Burden of Respiratory Syncytial Virus Infection in the Young and the Old, 2019 2019 0
47 M Ijaz, AB Siddique, MH Rasool, M Shafique Frequency of multi drug resistant Pseudomonas aeruginosa in different wound types of hospitalized patients Pak. J. Pharm. Sci. 32 (2), 865-870, 2019 865-870 2019 32 0
48 MUHAMMAD IJAZ, ABU BAKER SIDDIQUE, MUHAMMAD HIDAYAT RASOOL, MUHAMMAD SHAFIQUE Frequency of multi drug resistant Pseudomonas aeruginosa in different wound types of hospitalized patients Pakistan Journal of Pharmaceutical Sciences 865-870 2019 32 .804
49 A Mehmood, MH Rasool, AA Anjum, MA Zahoor, M Shafique Pathotyping and Genetic Characterization of a Highly Virulent Newcastle Disease Virus Strain Isolated from Recent Outbreak in District Okara, Pakistan. Pakistan Veterinary Journal 39 (3), 2019 2019 39 0
50 RF Alvi, B Aslam, N Shahzad, MH Rasool, M Shafique Molecular basis of quinolone resistance in clinical isolates of Klebsiella pneumoniae from Pakistan. Pakistan Journal of Pharmaceutical Sciences 31, 2018 2018 31 0
51 MUHAMMAD HIDAYAT RASOOL, AHSAN RAFIQ, MUHAMMAD ZEESHAN NAWAZ,MUHAMMAD SHAFIQUE AND MUHAMMAD SAQALEIN Sero-epidemiology and Risk Factor Analysis of Measles Among Children in Pakistan Polish Journal of Microbiology 227-231 2018 67 .75
52 ROMAN FAROOQ ALVI, BILAL ASLAM, NAVEED SHAHZAD, MUHAMMAD HIDAYAT RASOOL, MUHAMMAD SHAFIQUE Molecular basis of quinolone resistance in clinical isolates of Klebsiella pneumoniae from Pakistan Pakistan Journal of Pharmaceutical Sciences 1591-1596 2018 31 .804
53 RABIA QURESHI, MUHAMMAD SHAFIQUE, MUHAMMAD SHAHID, MUHAMMAD HIDAYAT RASOOL, SAIMA MUZAMMIL Molecular detection of blaVIM metallo-?-lactamase producing clinically isolated Pseudomonas aeruginosa from tertiary care hospital, Faisalabad Pakistan Journal of Pharmaceutical Sciences 2673-2677 2018 31 .804
54 FASEEHA KHALID, ABU BAKER SIDDIQUE, ZEESHAN NAWAZ, MUHAMMAD SHAFIQUE, MUHAMMAD ASIF ZAHOOR, MUHAMMAD ATIF NISAR AND MUHAMMAD HIDAYAT RASOOL EFFICACY OF BACTERIOPHAGE AGAINST MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES Southeast Asian J Trop Med Public Health 1056-1062 2017 42 .773
55 MUHAMMAD SHAFIQUE, MUHAMMAD HIDAYAT RASOOL, MOHSIN KHURSHID Respiratory syncytial virus: an overview of infection biology and vaccination strategies Future Virology 297-313 2017 12 1.121
56 F Khalid, AB Siddique, Z Nawaz, M Shafique, MA Zahoor, MA Nisar, ... Efficacy of bacteriophage against multidrug resistant Pseudomonas aeruginosa isolates Southeast Asian J. Trop. Med. Public Health 48 (5), 1056-1062, 2017 1056-1062 2017 48 0
57 MUHAMMAD SHAFIQUE, TJARKO MEIJERHOF, JAN WILSCHUT, AALZEN DE HAAN Evaluation of an intranasal virosomal vaccine against respiratory syncytial virus in mice: effect of TLR2 and NOD2 ligands on induction of systemic and mucosal immune responses PLoS One e61287 2013 8 4.09
58 TOBIAS KAMPHUIS, MUHAMMAD SHAFIQUE, TJARKO MEIJERHOF, TOON STEGMANN, JAN WILSCHUT, AALZEN DE HAAN Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats Vaccine 2169-2176 2013 31 3.766
59 K Tobias, M Shafique, T Meijerhof, T Stegmann, J Wilschut, A De Haan Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats. Vaccine 31 (17), 2169-2176, 2013 2169-2176 2013 31 0
60 M Shafique, T Meijerhof, J Wilschut, A de Haan Evaluation of an intranasal virosomal vaccine against respiratory syncytial virus in mice: effect of TLR2 and NOD2 ligands on induction of systemic and mucosal immune responses PloS one 8 (4), e61287, 2013 e61287 2013 8 0
61 MUHAMMAD SHAFIQUE, JAN WILSCHUT, AALZEN DE HAAN Induction of mucosal and systemic immunity against respiratory syncytial virus by inactivated virus supplemented with TLR9 and NOD2 ligands Vaccine 597-606 2012 30 3.766
62 M Shafique, J Wilschut, A De Haan Induction of mucosal and systemic immunity against respiratory syncytial virus by inactivated virus supplemented with TLR9 and NOD2 ligands. Vaccine 30 (3), 597-606., 2012 597-606. 2012 30 0
63 M Shafique, N Ahmad, FR Awan, T Mustafa, M Ullah, JA Qureshi Investigating the concurrent presence of HCV in serum, oral fluid and urine samples from chronic HCV patients in Faisalabad, Pakistan Archives of virology 154, 1523-1527, 2009 1523-1527 2009 154 0
64 MUHAMMAD SHAFIQUE, NASIR AHMAD, FAZLI R AWAN, TANVEER MUSTAFA, MUJIB ULLAH, JAVED A QURESHI Investigating the concurrent presence of HCV in serum, oral fluid and urine samples from chronic HCV patients in Faisalabad, Pakistan Archives of Virology 1523-1527 2009 154 2.209
65 N Ahmad, M Asgher, M Shafique, JA Qureshi An evidence of high prevalence of Hepatitis C virus in Faisalabad, Pakistan Saudi medical journal 28 (3), 390, 2007 390 2007 28 0
66 NASIR AHMAD, MUHAMMAD ASGHER, MUHAMMAD SHAFIQUE, JAVED ANVER QURESHI An Evidence of High Incidence of Hepatitis C Virus in Faisalabad, Pakistan Saudi Medical Journal 390-395 2007 28 .62
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